Wondering whether the cells in that vial you just thawed will ever “come back” after 5 or 10 years storage in liquid Nitrogen.
Will you have to wait weeks to see anything grow or just be disappointed with a culture flask full of dead cells?
Make 2012′s New Year’s resolution that I will freeze my cells with CryoStor cell preservation/freezing medium.
Want more proof…
CryoStor versus other freezing media
Experimental studies in molecular biology involving transformion of Escherichia coli and Bacillus subtilis assume that the recombinant plasmids are stable. However bacteria stored in stab-cultures or on nutrient agar can encourage phenotypic changes in the transformed bacteria. Limiting growth media can promote plasmid loss by imperfect segregation of plasmids into daughter cells (segregational instability) or promote a reduction in bacteria plasmid copies (deamplification). Bacteria containing large-sized plasmids can have reduced fitness in nutrient-limiting culture conditions to promote segregational instability, plasmid rearrangement or a reduction in plasmid copy number (1).
Smith, M.A. and M.J. Bidochka. 1988 Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size. Can. J. Microbiol. 44(4):351–355.
BactStorage allows for the recovery of viable cells after more than 15 freezing and thawing
cycles and after at least 10 years storage at -70-80 oC.
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BioLife Solutions has developed the next generation hypothermic storage and cryopreservation media products that extend the shelf life and improve post preservation viability and performance of cells, tissues, and organs.
HypoThermosol®
Storage and Shipping Media
Proprietary, best-in-class serum-free, protein-free, GMP-grade storage/shipping media for cells, tissues, and organs
CryoStor®
Freeze Media Pre-Formulated with DMSO
Proprietary, serum-free, protein-free, GMP-grade freeze media for cells, tissues, and organs
BloodStor™
GMP-grade umbilical cord blood stem cell freeze media with 55% DMSO USP (w/v) and 5% Dextran-40 USP (w/v)
Coming in July, 2010, TanTec Biosystems will offer antibodies and antigens from Virostat. Virostat products can be used to detect a wide variety of viruses, fungi and bacteria by ELISA, immunofluorescence, Western Blot and immunohistochemistry.
TanTec Biosystems is now offering Herpes virus prodcuts from Virusys. You can find a wide variety of antibodies and antigens for Herpes simplex virus I & II, Cytomegalovirus, Epstein-Barr virus, and Varicella Zoster virus.